Vol. 6, Issue 2, Part A (2024)

Isoflavones such as genistein and daidzein are increasingly incorporated into poultry feed formulations due to their phytoestrogenic, antioxidant, and growth-modulating properties. Accurate and rapid quantification of these compounds in complex feed matrices is essential for quality control, regulatory compliance, and feed-to-food safety evaluations. However, mixed chicken feed presents analytical challenges because of heterogeneous ingredients, variable fat and protein content, and potential co-extractives that interfere with detection. This research proposes a rapid and optimized analytical workflow combining UV-visible spectrophotometry and high-performance liquid chromatography (HPLC) for the quantification of genistein and daidzein in mixed chicken feed. Method optimization focused on solvent selection, extraction time, wavelength optimization for UV-Vis screening, and chromatographic conditions for HPLC confirmation, including mobile phase composition, flow rate, and detection wavelength. The UV-Vis method was optimized as a preliminary screening tool to enable rapid estimation of total isoflavone content, while HPLC served as a confirmatory and quantitative technique with higher specificity. Validation parameters such as linearity, precision, recovery, and repeatability were evaluated to ensure analytical robustness. The proposed approach emphasizes speed, reproducibility, and suitability for routine laboratory application without reliance on highly sophisticated instrumentation. By integrating UV-Vis and HPLC methodologies, the workflow balances analytical efficiency with quantitative accuracy. This method is intended to support feed manufacturers, researchers, and regulatory laboratories in monitoring isoflavone levels across diverse feed formulations. The research also provides a framework for adapting similar rapid analytical strategies to other bioactive compounds in animal feed matrices. Overall, the optimized UV-Vis/HPLC approach offers a practical solution for high-throughput isoflavone analysis while maintaining scientific rigor and reliability in mixed chicken feed assessment.