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International Journal of Chemical Research and Development

Vol. 5, Issue 2, Part A (2023)

Protein profiling and physico-chemical properties of Acacia nilotica Linn. gum exudates collected from different agro-climatic zones in India

Author(s):

Shruti Baghel, Ritu Rani Chaudhary, Ravi Prakash and RB Singh

Abstract:

Protein profiling and physico-chemical properties of seventeen Acacia nilotica Linn. (babool) gum exudates collected from ten Indian States covering five agro- climatic zones of India were studied to find out the intra-specific variations in their moisture level, total color difference (ΔE), yield %, ash content, elemental level, tannin content (mg/g), specific rotation [ α ], swelling index (%, v/v), viscosity and FT-IR. All the gum exudates showed Newtonian behavior with viscosity being shear rate independent. Gums were thermally characterized using differential scanning calorimetry (DSC), thermo-gravimetric analysis (TGA) and differential thermal analysis (DTA). DSC thermogram of gum samples showed presence of a broad endothermic peak at around 90-130 °C and occurrence of glass transition temperature in the range of 60.82-68.62 °C. TGA analysis showed three phases of weight loss indicating that the gum is thermally relatively stable. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of gum samples showed presence of mainly low molecular weight proteins. The observed intra-specific variations in the properties of Acacia nilotica gum exudates collected from different agro-climatic zones of India are presumably due to the effects of difference in their geographical locations, edaphic conditions, climatic factors, botanical sources, season and the age of the tree etc.

Pages: 37-40  |  82 Views  20 Downloads

How to cite this article:
Shruti Baghel, Ritu Rani Chaudhary, Ravi Prakash and RB Singh. Protein profiling and physico-chemical properties of Acacia nilotica Linn. gum exudates collected from different agro-climatic zones in India. Int. J. Chem. Res. Dev. 2023;5(2):37-40. DOI: 10.33545/26646552.2023.v5.i2a.54
International Journal of Chemical Research and Development

International Journal of Chemical Research and Development