Balanites aegyptiaca Linn. Plant leaves has been reported to use as traditional folk medicines especially in Africa and southern Asia in the form of juice to treat diarrhea, curing anthrax, dysentery, cuts and clean malignant wounds; whereas other parts of this plant have also reported their own traditional medicinal activities. This screening study is intended to evaluate the phytochemical ingredients and antioxidant activity of Balanites aegyptiaca Linn. Leaves aqua-ethanol extract with qualitative analysis method according to standard protocol and LC-MS/MS analysis. Antioxidant activity of aqua-ethanol extract was found to be significant with IC50 value 34.15±0.03 g/mL using DPPH (2, 2-Diphenyl-1-picryl hydrazyl) free radical scavenging assay method. The qualitative analysis of Balanites aegyptiaca Linn. Leaves aqua-ethanol extract revealed that the plant contain carbohydrate, protein, amino acids, glycoside, tannins, saponin, flavonoids and phenolic compounds. The LC-MS/MS analysis was carried out for the detection of bioactive phytochemical ingredients in the extract and reported 8 phytochemical ingredients, which are responsible for antioxidant activity. The results obtained in the present screening study indicate that Balanites aegyptiaca Linn. Leaves aqua-ethanol extract content promising free radical scavenging bioactive ingredients. Hence plant content promising bioactive ingredients responsible to antioxidant activity and treating diseases related to free radical reactions in reducing of oxidative stress related complications.
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