Vol. 5, Issue 1, Part A (2023)

Linum usitatissimum L a member of Linaceae family, which is used for various medical purposes. 
The aims of this study is the isolation of oil, testing with some microbes and measure some physicochemical properties. The seeds of Linum usitatissimum L was collected from the Omdurman and air-dried at room temperature, the oil extract of seed was prepared using soxhlet extraction with n-hexane, and analyzed using GC-MS Spectroscopy. The oil extract was tested for antimicrobial activity against 6 stander human pathogens Pseudomonas aeruginosa (P.s), Bacillus subtilis (B.s), Staphylococcus aureus (S.a), Escherichia coli (E.C), Condidaalbacans (Ca), and Aspergillusniger (A.s), and measured some physicochemical properties such as acid value, saponification value, and peroxide value. GC-MS characterization of fatty acid profile revealed the dominant fatty acid in 9,12,15-Octadectrienoic acid (51.46%), followed by oleic acid 9,12-Octadecdienoic acid(21.40%), Hexadecenoic acid (12.61%) and Methyl stearate (10.14%). Approximate of physiochemical properties of extracted oil revealed acid value (0,810 mg KOH/g of oil), saponification value (188.7), and peroxide value (0.99 meqO2/kg). The oil showed in active on all the test organisms except Candida albicans.