Supraja B, Sarath Babu N, RamaChandra B, Venkatasubba Naidu N
The definite, systematic, precise, particular, linear, proper and robust scientific method was developed and validated for the assay of Vinclozolin in Ronilan DF fungicide. Presently utilized Vinclozolin as a working standard having limit for assay of Vinclozolin in Ronilan DF fungicide are not less than 95.0% for method development and validation. The quantitative determination bring to accomplished by HPLC- Waters - Alliance 510 system equipped with UV/PDA detector. Methanol, Phosporic Acid and water in the ratio (55:45:0.1 v/v/v) used as mobile phase and flow rate 1.0 ml / min. with 15 minutes run time. The detection was carried at 220 nm with Newcrom R1 - 100mm x 3.2mm x 3µ column and ambient column temperature was maintained. In this connection, method uses the 20 μl injection volume and diluent as a blank solution. The linearity of this method was found to be linear in the range of 50% to 150% of the working concentration and the range for the analytical Method is 25 ppm to 75 ppm. The accuracy and precision of the method were within acceptable. The present developed HPLC method is detected to be suitable. The analytical solution was detected to be stable up to 48 Hrs at room temperature.
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